Actin gal4 driver

All GAL4 Drivers in the BDSC Collection. All GAL4 insertions at Bloomington known to be useful drivers of UAS-mediated expression are listed here. To limit the number of results displayed in the table below, you can navigate to GAL4 regulated by: The GAL4 expression information is provided by the donor of the stock. The driver line exhibits tissue-, and/or temporally, restricted expression of the yeast transcriptional activator GAL4. In most cases, GAL4 has no biological activity in insect cells, although in some cases, such as high-level expression in the retina, using GMR-GAL4, may cause mild artifactual phenotypes, possibly due to aggregation of the GAL4 at high . A nice very restricted driver would be pdf-gal4, but that list is virtually endless. Cite. All Answers (7) Spanish National Research Council. Actin-Gal4 could be too general, maybe you should Estimated Reading Time: 5 mins.

Act-GAL4/CyO is a 'workhorse' driver used in Drosophila labs throughout the world, the CyO balancer effectively precludes any HR events, else problems with this line would have occurred and been. neural elav-GAL4 or the ubiquitous actin-GAL4 drivers. Immunocytochemistry and Western blot confirmed that GAL4 drivers could specifically up-regulate the expression of the endogenous hsp22 gene in the absence of stress either in the nervous system as shown in EP(3)/elav-GAL4 embryos (Fig. 1B) or ubiquitously in EP(3)/ctina-GAL4 as observed. Using a UAS-lifeact-GFP transgene and the epidermal specific e22c-Gal4 driver, we labeled actin filaments in the larval epidermis. Actin was present at similar levels and was evenly distributed in the cytoplasm of unwounded epidermal cells in both control and UAS-chic IR(R3,R4)-expressing larvae (Fig. 4A,B).

Transglutaminase, when expressed with an actin driver, localizes to clot fibers differently from when expressed with a hemocyte driver. 1. Data. The data. fly using a ubiquitous Actin-Gal4 driver resulted in significant attenuation of TotM. expression in response to actin injection (Fig. 4a). Phalloidin staining of F-actin highlights muscle cells. Recognizable muscles are indicated with their corresponding number. B. Single channel image of (A).